DELFIA TRF Assays
DELFIA® (dissociation-enhanced lanthanide fluorescent immunoassay) TRF technology combines the benefits of time resolved fluorescence (TRF) with the use of lanthanide chelates for a highly sensitive, robust and reproducible assay format. With over 20 years of history, and thousands of publications, DELFIA is ideal for simple to complex matrices and can be used for both biochemical and cell-based assays.
The use of long-lifetime fluorescent lanthanide chelates, rather than other fluorophores, allows for a delay in measurement between excitation and emissions, which occur simultaneously in standard fluorometric assays. By combining the benefits of TRF with the use of lanthanide chelates, DELFIA offers multiple advantages over standard fluorometric detection:
- High sensitivity (femtogram level detection)
- Wide dynamic range (4-5 log)
- High S:B
- Large stokes shift
- Superior stability – (days)
- Multiplexing capability
From immunoassays, to binding and enzymatic studies, to cytotoxicity and cell proliferation assays, our DELFIA TRF assays are sure to provide you with unmatched sensitivity, time and time again.
For research use only. Not for use in diagnostic procedures.
Immunoassays
DELFIA delivers lower detection limits in the femtogram range, sensitivities that are at least 10x greater than standard colorimetric ELISA. DELFIA also offers a broad dynamic range, minimizing the need for time-consuming sample preparation and assay repeats.
DELFIA delivers lower detection limits in the femtogram range, sensitivities that are at least 10x greater than standard colorimetric ELISA. DELFIA also offers a broad dynamic range, minimizing the need for time-consuming sample preparation and assay repeats.
Non-radioactive cytotoxicity and cell proliferation assays
DELFIA cytotoxicity and cell proliferation assays provide an alternative to traditional radioactivity-based assays without compromising sensitivity or reliability. The DELFIA cytotoxicity assay closely mimics the traditional 51Cr-release protocol while the DELFIA Cell Proliferation assay couples 5-bromo-2-deoxyuridine (BrdU) incorporation with highly sensitive Europium-based TRF detection:
- Sensitive
- Time-saving
- Convenient
- Non-radioactive
DELFIA cytotoxicity and cell proliferation assays provide an alternative to traditional radioactivity-based assays without compromising sensitivity or reliability. The DELFIA cytotoxicity assay closely mimics the traditional 51Cr-release protocol while the DELFIA Cell Proliferation assay couples 5-bromo-2-deoxyuridine (BrdU) incorporation with highly sensitive Europium-based TRF detection:
- Sensitive
- Time-saving
- Convenient
- Non-radioactive
Binding assays
DELFIA provides a non-radioactive alternative to measure ligand-binding assays with Europium-labeled ligands, or biotinylated ligands in conjunction with Europium-streptavidin, using whole cells in adherent or filtration formats. It provides the sensitivity of traditional radioactive assays without the risk of exposure. DELFIA also offers a flexible, highly sensitive wash-based assay for protein-protein and other binding interactions that greatly reduces the potential for compound interference.
DELFIA provides a non-radioactive alternative to measure ligand-binding assays with Europium-labeled ligands, or biotinylated ligands in conjunction with Europium-streptavidin, using whole cells in adherent or filtration formats. It provides the sensitivity of traditional radioactive assays without the risk of exposure. DELFIA also offers a flexible, highly sensitive wash-based assay for protein-protein and other binding interactions that greatly reduces the potential for compound interference.
Enzymatic assays
Enzymes are important targets in drug discovery. Many marketed drugs today function through inhibition of enzymes mediating disease phenotypes. DELFIA provides a time-resolved fluorescence enzymatic assay compatible with peptide, protein, histone, nucleosome, and other substrates for biochemical assays, or fixed and live cells for cell-based assays. Furthermore, using DELFIA, multiplexing is possible.
Enzymes are important targets in drug discovery. Many marketed drugs today function through inhibition of enzymes mediating disease phenotypes. DELFIA provides a time-resolved fluorescence enzymatic assay compatible with peptide, protein, histone, nucleosome, and other substrates for biochemical assays, or fixed and live cells for cell-based assays. Furthermore, using DELFIA, multiplexing is possible.
DELFIA custom services
Are you working on a novel target? If you are looking to develop your own assay custom labeling services can conjugate your antibody or biomolecule to any of our DELFIA TRF chelates. Or maybe you are you short on time and need an assay developed quickly? Custom DELFIA assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your antibody or biomolecule custom-labeled or in developing a custom assay, please contact our custom team.
Are you working on a novel target? If you are looking to develop your own assay custom labeling services can conjugate your antibody or biomolecule to any of our DELFIA TRF chelates. Or maybe you are you short on time and need an assay developed quickly? Custom DELFIA assay development allows our technical experts to develop a custom assay just for your research needs. If you are interested in having your antibody or biomolecule custom-labeled or in developing a custom assay, please contact our custom team.
How can we help you?
When it comes to developing assays or troubleshooting issues, always know you’re never on your own. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.
FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.
Application support knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more.
When it comes to developing assays or troubleshooting issues, always know you’re never on your own. Whether you are looking to speak to someone directly or find the answers yourself we have the resources you need.
FAS: Our global team of Field Application Scientists are hands-on subject matter experts in assay development. They are available to assist in both assay development and troubleshooting.
Application support knowledgebase (ASK): Need help at 1am? We’ve got it covered. The Application Support Knowledgebase (ASK) contains detailed information on assay development, protocols, tips, FAQs, citations, troubleshooting and more.
Questions?
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